Plasmid

Part:BBa_K5136046:Design

Designed by: Xiaoxiao Zhang   Group: iGEM24_XMU-China   (2024-09-14)


pSB4A5 (with pColE408-aca2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3877
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3877
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3883
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3877
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3877
    Illegal XbaI site found at 3892
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

synthesis

References